Hepatoprotective, Antioxidant And Anti- Inflammatory Potential Of Lentinula Edodes Extract Against Diclofenac-Induced Hepatic Damage In Wistar Rats
Author(s): G. Sravan Kumar Reddy*, B.V.V. Ravi Kumar, M. Surya Prabha, Uppara Venkatesh, Ramu Samineni and G. Mary Ratna Anitha
Abstract
Lentinula edodes has traditionally been used to treat ulcers, bed sores, scalds and burns, hepatitis, gastritis, cancer, fungal infections, bronchial inflammation, and diabetes in Indian medicine, but its efficacy is unknown. Folklore, on the other hand, has given us numerous effective plant-based therapies. As a result, there are claims that Lentinula edodes (Omphalotaceae family) can be used to treat inflammation and hepatitis. The current study examined at the hepatoprotective, antioxidant, and anti-inflammatory properties of an alcoholic extract of Lentinula edodes. These fungi were able to inhibit the production of free radicals caused by the metabolism of the toxic substance diclofenac sodium. It also decreased diclofenac sodium-induced increases in Serum Aspartate Amino Transferase (AST), Alanine Amino Transferase (ALT), Alkaline Phosphatase (ALP), and Total Serum Bilirubin (SB), while increasing Lipid Peroxidation (LPO) and Glutathione (GSH) activities in the liver. A histopathological examination of the liver was performed to supplement the evidence from the biochemical analysis. The anti-inflammatory activity of carrageen was used to inhibit paw edoema. The results showed that the alcoholic extract of Lentinula edodes significantly (p<0.005) inhibited the carrageenan-induced paw edoema model, while the organic solvent extract (p<0.05 at 3rd h and 4th h) and the standard (p<0.01 at 3rd and p<0.001 at 4th h) when compared to the negative control. The presence of bioactive compounds such as flavonoids, tocopherol, and polyphenols reveals that Lentinula edodes has a significant protective effect against diclofenac-induced inflammation and acute hepatotoxicity
