M. Mukkanti Eswarudu

Department of Pharmacy, Jawaharlal Nehru Technological University, India

Publications

• Research Article   
  Novel Validated LC-MS/MS Method for Simultaneous Estimation of Celecoxib and Amlodipine in Rat Plasma and its Application to a Pharmacokinetic Study
  Author(s): M. Mukkanti Eswarudu, A. Lakshmana Rao* and K. Vijay
  
  The combination of celecoxib (CLX) and Amlodipine (AMD) was approved for hypertensive patients with osteoarthritis by US-FDA. Hence, a potential analytical method that can simultaneously quantify these two drugs is required. In view of this, a novel and fully validated liquid chromatography-electrospray ionization-tandem mass spectrometric (LC-ESI-MS/MS) method has been established for the quantification of CLX and AMD in rat plasma simultaneously. Protein precipitation extraction technique was employed for the extraction of analytes and their deuterated analogues from rat plasma quantitatively. The analytes were separated using the mobile phase comprising of acetonitrile–water with 0.1% formic acid buffer (70:30 v/v) and a flow-rate of 1.0 mL/min and 10 minutes run time on Agilent SB-C18 analytical column. The multiple reaction monitoring tra.. Read More»
  DOI: 10.4303/JDAR/236214

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